human glioma cell lines hs683  (ATCC)

Journal: Discover Oncology

Article Title: Identification and validation of LDHA and SLC16A1 for predicting prognosis and diagnosis in lower-grade glioma

doi: 10.1007/s12672-025-03297-2

Figure Lengend Snippet: Expression of SLC16A1 in SW1088 and HS683 after transfection with si-SLC16A1. A The expressions of LDHA and SLC16A1 in low-grade glioma cell lines. The X-axis represented different cells, while the Y-axis represented the relative expression of LDHA and SLC16A1. Control, normal human glial cells; Low-grade glioma cell lines SW1088 and HS683. **, P < 0.01, when compared with control. B The mRNA expression of SLC16A1 was detected by qRT-PCR. C The protein expression of SLC16A1 was detected by western blot. Protein concentrations were determined by BCA assay, and equal amounts (30 µg) were loaded in each lane.***, P < 0.001

Article Snippet: The LGG cell lines of SW1088 and HS683 cell lines were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Transfection, Control, Quantitative RT-PCR, Western Blot, BIA-KA

Journal: Discover Oncology

Article Title: Identification and validation of LDHA and SLC16A1 for predicting prognosis and diagnosis in lower-grade glioma

doi: 10.1007/s12672-025-03297-2

Figure Lengend Snippet: Low expression of SLC16A1 inhibits glioma cell proliferation, migration, and invasion. A Proliferation curve of SW1088 cells. B Proliferation curve of HS683 cells. C Transwell assay was used to detect the migration ability of SW1088 and HS683 cells (×100). D Transwell assay was used to detect the invasion ability of SW1088 and HS683 cells (×100). *, P < 0.05, **, P < 0.01, ***, P < 0.001

Article Snippet: The LGG cell lines of SW1088 and HS683 cell lines were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Migration, Transwell Assay

Journal: Journal of Korean Neurosurgical Society

Article Title: Spermine Synthase : A Potential Prognostic Marker for Lower-Grade Gliomas

doi: 10.3340/jkns.2024.0080

Figure Lengend Snippet: Knockdown of spermine synthase (SMS) inhibits the malignant behavior of lower-grade glioma cells. A : Western blot analysis of SMS protein expression levels in human normal glial cell lines CP-H121/122/123 and glioma cell line HS683/U251, with statistical analysis on the right. B : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by Western blot, with protein expression analysis on the right. C : The efficiency of SMS knockdown in HS683/U251 glioma cell line detected by quantitative reverse transcription polymerase chain reaction. D : Cell proliferation before and after SMS knockdown was measured by CCK-8 assay. E : The apoptosis ratio of cells before and after SMS knockdown was measured by flow cytometry, with statistical analysis on the right. F : Several cell clones formed before and after SMS knockdown, with statistical results. G : Migration of cells before and after SMS knockdown measured by scratch wound healing assay. H : Migration and invasive ability of cells before and after SMS knockdown measured by transwell assay. NC : normal control. *** p <0.001.

Article Snippet: The HS683 (HTB-138) human oligodendroglioma cell line and the U251 (HTB-17) glioblastoma cell line were obtained from ATCC (Manassas, VA, USA).

Techniques: Knockdown, Western Blot, Expressing, Reverse Transcription, Polymerase Chain Reaction, CCK-8 Assay, Flow Cytometry, Clone Assay, Migration, Wound Healing Assay, Transwell Assay, Control

Journal: Annals of Medicine and Surgery

Article Title: Unraveling the role of PBK in glioblastoma: from molecular mechanisms to therapeutic targets

doi: 10.1097/MS9.0000000000002708

Figure Lengend Snippet: Association between EIF4E and PBK and patient survival. The co-expression relationship between EIF4E and PBK was performed by the cBioPortal online platform (A). The expression of EIF4E was downregulated after PBK knockdown in HS683 (B). GEPIA online platform was used to perform the expression of EIF4E in glioma and its effect on survival. EIF4E mRNA expression in GBM compared to normal brain tissues (C) in which normal samples are marked in gray and GBM samples are marked in red. High EIF4E expression decreases both overall survival (D) and disease-free survival (E).

Article Snippet: The human glioma cell line HS683 was obtained from the American Type Culture Collection (ATCC) and cultured according to ATCC instructions in DMEM medium with 10% FBS in a 5% CO 2 incubator at 37°C. shRNA sequences for PBK knockdown and cell line establishment were based on a previous study .

Techniques: Expressing, Knockdown

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: ( A ) The qRT-PCR assays validated the siRNA knockdown effect. ( B ) The results of CCK-8 assay in HS683 cell lines. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: Quantitative RT-PCR, Knockdown, CCK-8 Assay

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: Scratch wound healing assessment of HS683 cell lines following treatment with siRNA or negative control (NC) targeting four hub genes: ( A ) CDCA8, ( B ) DLGAP5, ( C ) ORC1 and ( D ) SMC4. Transwell examination of LGG cell lines treated with siRNA or negative control (NC) to target four hub genes: ( E ) CDCA8, ( F ) DLGAP5, ( G ) ORC1 and ( H ) SMC4. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: Negative Control

Journal: Scientific Reports

Article Title: Stemness subtypes in lower-grade glioma with prognostic biomarkers, tumor microenvironment, and treatment response

doi: 10.1038/s41598-024-65717-7

Figure Lengend Snippet: The results of CCK-8 assay in HS683 cell lines. ( A ) SMC4, ( B ) DLGAP5, CDCA8 and ( D ) ORC1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, no significance.

Article Snippet: The HS683 human glioma cell line was sourced from Wuhan Procell Life Science and Technology Co., Ltd. (Procell, China).

Techniques: CCK-8 Assay